Genotypic and Allelic Frequencies of IGF1 and IGF2 Genes in Broilers Analysed by Using PCR‐RFLP Techniques

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test was performed to investigate the allele frequencies of the insulin-like growth factor (IGF1 and IGF2 genes) in 90 broilerchickens. A 793 bp fragment of IGF1 gene and 1146 bp fragment of IGF2 gene were amplified and digested with HinfI and Hsp92II restriction enzymes,respectively. Two types of alleles of A and B and three types of genotypes of AA, AB and BB were observed in both genes. The results indicated predominant B-allele (0.85) was higher for IGF1 butA-allele (0.5611) IGF2 in the broiler population.